Acromioclavicular joint injuries are common, especially among athletes. Multiple techniques have been described for the reconstruction of acromioclavicular joint separation. Techniques described earlier or currently have addressed the coracoclavicular and acromioclavicular reconstruction, addressing only the superior-inferior and anteroposterior instability components. We describe an arthroscopy-assisted global and biological reconstruction of subacute acromioclavicular joint separation using a hamstring graft, showing the reconstruction of coracoclavicular, acromioclavicular, and coracoacromial ligaments and the deltotrapezial fascia to address superior-inferior instability, anteroposterior instability, and scapular dyskinesia.